Bacterial Cell Biology
Prof. Dr. Marc Bramkamp
Dr. Karin Schubert
Practical course in combination with a seminar.
Bacterial cells have a high degree of internal organization. Hence, macromolecules are often precisely localized to subcellular compartments. In this course we will use genetic tools to construct fluorescent fusions to cytokinetic or cytoskeletal proteins and use fluorescence microscopy to analyze protein localization and dynamics in vivo. Further, these proteins will be purified using fast protein liquid chromatography and their function will be analyzed in vitro using classical biochemical methods.