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Topics for Master Theses (Group H. Jung)

1. Significance and function of membrane transport proteins at the example of PutP and CaiT

Organism: Escherichia coli, Helicobacter pylori

Methods: site-directed mutagenesis, electrophoretic techniques, affinity chromatography, gel filtration, solubilization and reconstitution of membrane proteins, Western Blot, transport experiments, site-directed protein labeling, fluorescence spectroscopy


2. Evolution of cooperativity in bacterial populations

Organism: Pseudomonas putida

Methods: diverse microbial cultivation techniques, generation and analysis of structured populations, fluorescence spectroscopy, fluorescence microscopy


3. Phenotypic heterogeneity of siderophore production in pseudomonads

Organism: Pseudomonas putida

Methods: genetic engineering, generation of reporter gene fusions, fluorescence microscopy, single cell analysis, time lapse microscopy