Topics for Master Theses (Group H. Jung)
1. Significance and function of tripartite efflux pumps
Organism: Pseudomonas putida
Methods: diverse phenotypic analyses, site-directed mutagenesis, electrophoretic techniques, affinity chromatography, gel filtration, solubilization and reconstitution of membrane proteins, Western Blot, transport experiments, fluorescence spectroscopy
2. Mechanisms of signal transduction in pseudomonads
Organism: Pseudomonas putida
Methods: genetic engineering, generation of reporter gene fusions, fluorescence microscopy, single cell analysis, time lapse microscopy, protein purification and functional characterization